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KMID : 0381120130350010087
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Genes and Genomics
2013 Volume.35 No. 1 p.87 ~ p.93
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Gene cloning and functional analysis of yellow green leaf3 (ygl3) gene during the whole-plant growth stage in rice
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Tian Xiaoqing
Ling Yinghua
Fang Likui
Du Peng
Sang Xianchun
Zhao Fangming
Li Yunfeng
Xie Rong
He Guanghua
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Abstract
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Chlorophyll is an important photosynthetic pigment in the process of photosynthesis in plants and photosynthetic bacteria. Genes involved in chlorophyll biosynthesis in Arabidopsis and photosynthetic bacteria have been well documented. In rice, however, these genes have not been fully annotated. In this paper, a yellow-green leaf gene, yellow green leaf3 (ygl3) was cloned and analyzed. ygl3 encodes magnesium chelation ChlD (D) subunit, a key enzyme for chlorophyll synthesis, resulting in a yellow-green leaf phenotype in all growth stages in rice. Expression content of ygl3 is highest in the leaf blades, followed by the leaf sheaths, while there is virtually no expression of the gene in the stems and seeds. The sub-cellular structure and protein content of the photosynthetic system of the ygl3 mutant were revealed by transmission electron microscopy, BN-PAGE, and western blotting. The results show that the mutation of the ygl3 gene indirectly leads to a decrease in the protein content of the photosynthetic system and severely obstructs the formation of granum thylakoids.
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KEYWORD
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Rice, Yellow green leaf 3, Ygl3, RNAi
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